mTOR Inhibition by Everolimus in Childhood Acute Lymphoblastic Leukemia Induces Caspase-Independent Cell Death
Figure 4
(A) NALM6 cells were treated with 16 µM everolimus overnight and qRT-PCR used to evaluate the expression of the indicated genes. The mean ± SD of the fold change from 3 experiments is shown. (B) NALM6 cells were incubated with the indicated concentrations of everolimus for the specified time periods and cell lysates prepared. Sequential Western blotting was performed on the same membrane to determine the levels of the indicated proteins in each series of panels. Phospho-eIF2α bands were quantitated by densitometry and normalized to β-actin.