Quantifying Metabolic Heterogeneity in Head and Neck Tumors in Real Time: 2-DG Uptake Is Highest in Hypoxic Tumor Regions
Figure 5
Heterogeneous staining pattern of HIF-1α, LDH-M, and CAIX expression in xenograft tumors reflects presence of hypoxia.
Representative images of HypoxiSense Cal33 tumor sections were probed for HIF-1α, LDH-M, and CAIX expression by immunohistochemistry (IHC) as described in materials and methods. Gray boxes indicate the region of the tumor shown at 10× magnification. Slides were scanned at 20× on Aperio Imagescope software. Images of whole tumor sections were captured at 20×. Images of regions within tumor slices were captured at 200×. Additional inset of CAIX shows cell-surface staining of this protein in peri-necrotic areas.