The Histone Acetyltransferase p300 Regulates the Expression of Pluripotency Factors and Odontogenic Differentiation of Human Dental Pulp Cells
Figure 5
p300 increases the activities of the NANOG and SOX2 promoters.
(A) HDPCs were transiently transfected with 500 ng of the NANOG or SOX2 promoter construct and 5 µg of the p300 or p300-ΔHAT expressing vector. Cells were harvested and the activities of the promoter were measured after 24 hours of transfection. (B) HDPCs were transiently transfected with 500 ng of the NANOG or SOX2 promoter construct and increasing amounts (0–5 µg) of the p300-expressing vector. After 24 hours of transfection, the cells were harvested and the activities of the promoters were measured. Procedures were performed as described in the text (n = 3). All results are presented as the means ± SD of three independent experiments. * Statistically significant difference relative to the control, P<0.05.