Up-Regulation of MicroRNA-145 Associates with Lymph Node Metastasis in Colorectal Cancer
Figure 5
Knockdown of Hsp-27 by siRNA attenuated the prometastatic effect of miR-145.
(A) HCT-8-mir-145 (mir-145) or HCT-8-NC (NC) cells were transfected with Hsp-27 siRNA (mir-145+si-hsp27) or a negative control siRNA (mir-145+mock). The expression of Hsp-27 protein was detected by western blot assay. (B) Wound-healing assay to evaluate the effect of Hsp-27 siRNA in HCT-8-miR-145 cells. (C) Knockdown of Hsp-27 by siRNA in HCT-8-miR-145 cells significantly inhibited cell invasion. The images were representatives of at least three independent experiments. Average number of invasion cell number per field from at least three independent experiments ± SD is shown by column figure. ** P<0.01.