Norepinephrine Stimulates Mobilization of Endothelial Progenitor Cells after Limb Ischemia
Figure 12
NE activated EPCs via Akt/eNOS pathways.
Treatment of NE significantly increased phosphorylation of Akt, eNOS. Addition of 10 µM Phentolamine and 10 µM I127 attenuated the activation of Akt/eNOS pathway. However, addition of 10 µM Metorolol could not. Representative immunoblots (top) and densitometric quantification (bottom) demonstrated phosphorylation of Akt and eNOS in the following groups: control, 0.1 µM NE, 1 µM NE, 10 µM NE, 100 µM NE, NE plus Phentolamine, NE plus Metorolol and NE plus I127. *P<0.05 vs control.