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Norepinephrine Stimulates Mobilization of Endothelial Progenitor Cells after Limb Ischemia

Figure 11

Inhibition of α adrenoceptor and β 2 adrenoceptor attenuated the elevation of EPCs in spleen. Cells from splenic tissue homogenates were lysed and analyzed with flow cytometry.

EPCs in spleen were defined as CD34+/KDR+ cells (B). Co-treatment of either Phentolamine or I127 with NE significantly attenuated the increases of EPCs in spleen, but Metoprolol didn't (B, *P<0.05 compared with model group.). Representative flow cytometric analyses of EPCs (CD34+/Flk-1+cells) were showed in part B.

Figure 11

doi: https://doi.org/10.1371/journal.pone.0101774.g011