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Norepinephrine Stimulates Mobilization of Endothelial Progenitor Cells after Limb Ischemia

Figure 10

Inhibition of α adrenoceptor and β 2 adrenoceptor attenuated the elevation of EPCs in BM.

Cells from BM homogenates were lysed and analyzed with flow cytometry. BM derived EPCs were defined as CD34+/KDR+ cells (B). Co-treatment of either Phentolamine or I127 with NE significantly attenuated the increases of EPCs in peripheral circulation, but Metoprolol didn't (B, *P<0.05 compared with model group). Representative flow cytometric analysis of EPCs (CD34+/Flk-1+cells) were showed in part B.

Figure 10

doi: https://doi.org/10.1371/journal.pone.0101774.g010