Norepinephrine Stimulates Mobilization of Endothelial Progenitor Cells after Limb Ischemia
Figure 7
Proliferation potential of EPCs was increased by NE and blocked by Phentolamine, I127, LY294002 and L-NAME in vitro.
Growth capacity of PBMC-derived EPCs after 8 days of culture was evaluated (n = 6). As shown by multiwell spectrophotometer, the treatment of NE induced a significant increase of the number of EPCs in the S-phase in a dose-dependent manner, as well as proliferation of EPC (* P<0.05 compared with model group). Pretreatment of phentolamine, I127, LY294002 and L-NAME significantly blocked the effect of NE on EPCs proliferation (Figure 5), but Metoprolol could not.