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Norepinephrine Stimulates Mobilization of Endothelial Progenitor Cells after Limb Ischemia

Figure 3

NE increased EPCs in spleen in mice bearing limb ischemia.

Cells from splenic tissue homogenates were lysed and analyzed with flow cytometry. EPCs in spleen were defined as CD34+/KDR+ cells (B). Proportion of EPCs in spleen was increased from 1.94±0.39% to 4.89±0.36% after intraperitoneal injection of NE in mice with limb ischemia (A, * P<0.05 compared with model group). Representative flow cytometric analysis of EPCs (CD34+/Flk-1+cells) were showed in part B.

Figure 3

doi: https://doi.org/10.1371/journal.pone.0101774.g003