Norepinephrine Stimulates Mobilization of Endothelial Progenitor Cells after Limb Ischemia
Figure 1
NE increased EPCs in peripheral blood in mice with limb ischemia.
Cells from peripheral blood were lysed and analyzed by flow cytometry. Cells were sequentially gated based on CD45 (A), CD34 and KDR expression (C). Circulating EPCs were defined as CD45-/CD34+/KDR+ cells. A gate was used to select the total CD45- cell population (A). Corresponding flow cytometric analysis was used to detect CD34+/KDR+ cells in the gated CD45- cell population. Proportion of EPCs in peripheral blood was increased from 0.15±0.04% to 0.31±0.05% after intraperitoneal injection of NE in limb ischemia model (B, * P<0.05 compared with the model group). Representative flow cytometric analysis of EPCs (CD34/Flk-1 cells) were showed in part C.