A Small Molecule Inhibitor of Human RAD51 Potentiates Breast Cancer Cell Killing by Therapeutic Agents in Mouse Xenografts
Figure 6
B02 inhibits the RAD51 foci formation in tumor tissue.
NCR nude mice burdening with MDA-MB-231 xenograft tumors were treated by I.P. with either B02 (50 mg/kg), cisplatin (6 mg/kg), both or left untreated. The mice were sacrificed 3 h after administration, and tumor tissues were dissected and fixed in 10% formalin. Nuclei were counterstained with DAPI. After immunostaining with RAD51 antibodies, RAD51 foci were visualized using a ZEISS LSM5 confocal microscope with a 63× oil objective. Bars indicate 20 µm. The low panel shows the number of foci per nucleus in foci positive cells; it was determined by counting at least 20 nuclei in each experiment. Error bars represent SD.