Chimeric Avidin – NMR Structure and Dynamics of a 56 kDa Homotetrameric Thermostable Protein
Figure 5
Overlay of the NMR structure of biotin–bound ChiAVD and the X–ray structure of biotin–free ChiAVD.
(A) ChiAVD tetramer where the biotin–bound form is represented in dark blue and (B) biotin binding site residues. In (B) aromatic residues are shown in shades of blue (darker colors for the biotin–bound form) and polar residues in shades of green. The 1–2 interface brings Trp110 from the 1–2 related subunit to the binding site.