Role of TLR5 and Flagella in Bacillus Intraocular Infection
Figure 5
Influence of TLR5 on proinflammatory mediator expression during experimental B. cereus endophthalmitis.
C57BL/6J and TLR5−/− mouse eyes were injected with 100 CFU B. cereus. Ocular proinflammatory cytokines and chemokines were analyzed by sandwich ELISA. Overall, similar levels of TNFα, KC, IL6, and IL1β were synthesized in infected eyes of C57BL/6J mice compared with that in infected eyes of TLR5−/− mice, suggesting that the absence of TLR5 did not alter the inflammatory mediator response during infection. Values represent the mean ±SD for N≥4 per group for at least 2 separate experiments. *P≤0.05.