Efficient Induction of Apoptosis by Wee1 Kinase Inhibition in Hepatocellular Carcinoma Cells
Figure 2
Targeting Wee1 kinase induces apoptosis in HCC cells.
A, The number of sub-G1 HuH7 cells significantly increased after PD166285 treatment (200 nM). PD166285-mediated apoptosis of HuH7 cells was completely inhibited by pretreatment with 20 µM roscovitine (Ros). * indicates significant differences between each group (P<0.05). The results are presented as means ± S.D. of three experiments. B, PD166285 decreased the number of HuH7 cells in a concentration-dependent manner after 72 h. * indicates significant differences between each group (P<0.05). The representative results of three independent experiments are shown. C and D, Wee1 kinase-specific siRNAs (18-2 or 18-3) significantly reduced the total number of cells and increased the sub-G1 cell population 48 h after transfection in a concentration-dependent manner compared with control-vector transfectants. * indicates significant differences between each group (P<0.05).