Pannexin-1 Is Blocked by Its C-Terminus through a Delocalized Non-Specific Interaction Surface
Figure 5
Poly-alanine (pAla) substitutions did not result in a constitutively active Panx1 channel.
(A) HEK293T cells were transfected with a series of poly-alanine mutant Panx1 channels and assayed for Yo-Pro influx (example images shown). Scale bars are 100 µm. (B) All the poly-alanine mutants were expressed at the cell surface except for the outer tail-end substitution pAla-5, which did not express as determined by surface biotinylation-Western. (C and D) None of the poly-alanine mutants tested displayed any measurable Yo-Pro influx over time when compared with the Δ379 mutant. In particular, pairwise Student's T-test comparisons showed no statistically significant difference between mock, FL, and any of the poly-alanine mutants. (E) The c-terminal sequences of the poly-alanine mutants are as shown.