KIR2DS4 Promotes HIV-1 Pathogenesis: New Evidence from Analyses of Immunogenetic Data and Natural Killer Cell Function
Figure 1
KIR2DS4 gene expression and natural killer (NK) cell function in subjects with chronic HIV-1 infection.
A. Flow cytometry gating strategy for NK cells. B. Staining of membrane-bound KIR2DS4 on cells derived from subjects with full-length KIR2DS4 gene (g+), to facilitate the sorting of two populations positive (p+) or negative (p−) for the gene product. C. Staining of membrane-bound KIR2DS4 on cells derived from subjects with truncated KIR2DS4 gene only, i.e., negative for full-length KIR2DS4 gene and gene product (g−/p−). D. Percentage of NK cells expressing KIR2DS4 before and after stimulation with HLA-deficient target cells (K562 and 221). E. Distribution of NK cell subsets among 43 subjects with and without the full-length KIR2DS4 genotype. F–H. Representative results for staining cell surface CD107a (lysosomal-associated membrane protein 1) and intracellular IFN-γ or MIP-1β before (red) and after (blue) stimulation with K562 cells. In D and E, the horizontal bars connected by a vertical line correspond to the median and interquartile range.