Delayed Effects of Corticosterone on Slow After-Hyperpolarization Potentials in Mouse Hippocampal versus Prefrontal Cortical Pyramidal Neurons
Figure 5
The long lasting effects of corticosterone treatment on AHPs and action potential firing of layer 2/3 infralimbic pyramidal neurons.
A: left: a sample pyramidal neuron filled with fluorescent dye. Inset: zoomed image of the neuron (white arrow). Scale bar equals 20 microns. Right, locations of the recorded neurons on a pictorial coronal mouse brain section. B: representative AHP responses from vehicle and corticosterone groups overlaid with the fit. Light gray: raw trace, thick dark gray: fit, dotted line: mAHP decay, thin black line: sAHP decay. Inset shows the current protocol used to evoke the AHPs. C: traces of action potentials from neurons representative of the vehicle and corticosterone groups. Marking indicate the positions of spike amplitude, half-width and threshold measurements. D: mAHP peak amplitude averaged per group and per current step. E: averaged values of sAHP peak amplitude for each current injection step. F: accommodation index (% ratio of the interspike intervals of the last two spikes to the first two spikes) from vehicle and corticosterone groups. Number within brackets in the legends indicates number of neurons.