Delayed Effects of Corticosterone on Slow After-Hyperpolarization Potentials in Mouse Hippocampal versus Prefrontal Cortical Pyramidal Neurons
Figure 3
The long lasting effects of corticosterone treatment on AHPs and action potential firing of layer 2/3 lateral OFC pyramidal neurons.
A: locations of the recorded neurons on a pictorial coronal mouse brain section. B: representative AHP responses from vehicle and corticosterone groups overlaid with the fit. Light gray: raw trace, thick dark gray: fit, dotted line: mAHP decay, thin black line: sAHP decay. Inset shows the current protocol used to evoke the AHPs. C: traces of action potentials from neurons representative of the vehicle and corticosterone groups. Marking on the trace indicate the positions of spike amplitude, half-width and threshold measurements. D: mAHP peak amplitude averaged per group and per current step. E: averaged values of sAHP peak amplitude for each current injection step. F: Power of the sAHP interaction effect (corticosterone treatment x current steps) with increasing total sample size. Dotted vertical line indicates the sample size (∼70) required for a power of 0.8. Open square in the plot indicates the power at the actual sample size. G: accommodation index (% ratio of the interspike intervals of the last two spikes to the first two spikes) from vehicle and corticosterone groups. Number within brackets in the legends indicates number of neurons.