Loss of Sonic Hedgehog Leads to Alterations in Intestinal Secretory Cell Maturation and Autophagy
Figure 5
Enteroendocrine cells are not affected by the loss of intestinal epithelial Shh signaling.
Chromogranin A was used as a general endocrine cell marker as it labels all subtypes of enteroendocrine cells. No modulation was observed in chromogranin A (CgA)-positive cells (green staining) in the ileal epithelium of control (A) and ShhΔIEC mice (B). Evans blue (red staining) served as a counterstain. The number of enteroendocrine (CgA)-positive cells was determined from the ileum of control and mutant mice (n = 4) (C). Scale bar: 50 µm. Two-way ANOVA. Error bars represent SEM.