RETRACTED: Identification of Nitric Oxide as an Endogenous Inhibitor of 26S Proteasomes in Vascular Endothelial Cells
Figure 5
NO at high concentrations inhibits the activity of purified 26S proteasomes.
(A) SNP decreased the chymotrypsin-like activity of the purified 26S proteasome. (B) DETA-NONOate reduced the chymotrypsin-like activity of the purified 26S proteasome. The purified 26S proteasome (Enzo Life Sciences, Inc) were incubated with SNP or DETA-NONOate at indicated concentrations up to 1000 µM for 0.5 h followed by assay of the chymotrypsin-like activity. * represent p<0.05 vs control (n = 3). DETA-NONOate, Diethylenetriamine NONOate; SNP, sodium nitroprusside.