Metformin Induces Apoptosis and Cell Cycle Arrest Mediated by Oxidative Stress, AMPK and FOXO3a in MCF-7 Breast Cancer Cells
Figure 3
Metformin decreased the activation of insulin signaling pathway.
(A) A western blot of IRβ, p-IRβ (Tyr 1162/1163), Akt, p-Akt1/2/3 (Ser 413), ERK1/2 and p-ERK1/2 (Thr 202/Tyr 204) with β-actin as the control band. (B) Western blot ratio analysis of p-IRβ (Tyr 1162/1163) and IRβ, (C) p-Akt1/2/3 (Ser 413) and Akt and (D) p-ERK1/2 (Thr 202/Tyr 204)and ERK1/2 in MCF-7 cells after treatment with metformin (10 mM) for 24, 48 and 72 hours. * p<0.05 vs. control; ** p<0.001 vs. control.