Human Sarcoma Growth Is Sensitive to Small-Molecule Mediated AXIN Stabilization
Figure 3
SEN461 effects on Wnt molecular components in sarcoma cells.
(A, D) Effect of SEN461 or XAV939 on Wnt target genes mRNA expression measured by quantitative RT-PCR in U2OS and HT-1080 cells after 10 µmol/L treatment. Data (collected from three independent experiments) represent means ± SEM. **, P<0.05 ***, P<0.005 relative to control cells (Student t test). (B, E) Western blotting analysis of U2OS and HT-1080 cell lines treated with SEN461 or XAV939 overnight. Cytoplasmic cell lysates were probed with anti-Axin1, anti-TNKS1/2 and anti-Tubulin as loading control. (C, F, G) Western blotting analysis of U2OS and HT-1080 treated with SEN461 or XAV939 overnight. Cytoplasmic and nuclear cell lysates were then probed with anti-c-Myc, anti-p21 and anti-β-actin as loading control. The asterisk represents a background band migrating below the p21 band.