< Back to Article

Three Dimensional Fluorescence Imaging Using Multiple Light-Sheet Microscopy

Figure 5

Demonstration of multi-plane imaging capability.

(A) Schematic depiction of the experimental setup (not to scale). The near focal region of the cylindrical lens is magnified to show the multiple light-sheets. The orthogonal detection arm is traversed along the axis to focus on the different light-sheets. The numbers figuratively depicts five light-sheets from which experimental images were obtained. (B) Experimental images corresponding to different planes illuminated by the light-sheets. The sample used was fluorescently coated yeast cells encaged in agar gel matrix. Each image is parallel to the plane and contains images of yeast cells in focus and defocussed images of cells illuminated by other light-sheets. Some of the in-focus cells are marked by the orange boxes and those regions are zoomed and presented below each such image. The distance between each image () is approximately and the scalebar represents .

doi: https://doi.org/10.1371/journal.pone.0096551.g005