Three Dimensional Fluorescence Imaging Using Multiple Light-Sheet Microscopy
Figure 3
Experimental generation of multiple light-sheets and characterization.
(A) Schematic diagram of the experimental setup used for characterizing the illumination PSF. (B) Experimentally obtained transverse profile of the multiple light-sheet system. (C) Intensity plot of the experimentally obtained illumination PSF along y−axis. The inter sheet separation is found to be 15 whereas the FHWM of each sheet is 7.5
. The dotted line indicate
prominent light-sheets with relative intensity
.