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Three Dimensional Fluorescence Imaging Using Multiple Light-Sheet Microscopy

Figure 2

Illumination PSFs obtained from computational simulation.

The study was carried out for two configuration, small numerical aperture (aperture angle, , stop angle, ), and large numerical aperture (aperture angle, , stop angle, ). For comparison, the illumination PSF of the state-of-art SPIM system (aperture angle, , stop angle, ) is also shown. Scale bar is .

Figure 2

doi: https://doi.org/10.1371/journal.pone.0096551.g002