A Novel Antibody against Human Properdin Inhibits the Alternative Complement System and Specifically Detects Properdin from Blood Samples
Figure 5
(A) A model of mAb 1340 secondary structure shows the heavy (blue) and light (red) variable chain. The binding cleft is determined by six CDR loops (marked are overlapping amino acids from Table 1) either in the heavy chain H1 (orchid), H2 (salmon), H3 (magenta) or in the light chain L1 (dark green), L2 (chartreuse), L3 (springgreen). The distance between the chains varies between 11.66–24.64 Å. Modeling was performed with Rosetta. (B) Spherical display of mAb 1340 light (light grey) and heavy (dark grey) variable chain. The predicted amino acids for antigen contact are highlighted. The coloring of the residues is according to their contact probability value (the higher the probability the deeper the color). Modeling was performed with proABC.