Megakaryocytic Differentiation of K562 Cells Induced by PMA Reduced the Activity of Respiratory Chain Complex IV
Figure 6
Inhibition of respiratory chain complex IV activity decreased mitochondrial membrane potential.
(A) K562 cells were pre-treated with different doses of SA for 3 hour, and then the mitochondrial membrane potential (JC-1 staining) was determined by flow cytometry analysis. (B) K562 cells (3×105 cells/ml) were pre-treated with 5 µM CsA for 6 h and then treated with 5 nM PMA for the indicated time. The mitochondrial protein complexes were separated by hrCN-PAGE gel and then identified by in-gel catalytic activity assay of complex IV. (C & D) K562 cells were pre-treated with the complex IV specific inhibitor SA for 3 h, then cells were induced by 5 nM PMA for 72 h. Expression of CD41 and CD61 was determined by flow cytometry analysis. Data were shown as mean ± SD of three independent experiments, *p≤0.05, **p≤0.001.