Megakaryocytic Differentiation of K562 Cells Induced by PMA Reduced the Activity of Respiratory Chain Complex IV
Figure 3
Effects of PMA-induced megakaryocytic differentiation on mitochondrial morphology and ultrastructure.
1×106 K562 cells were incubated with 5 nM PMA and harvested at 12 h, 24 h and 72 h respectively, Cells labeled with NAO were detected by flow cytometry analysis and confocal microscopy (A, B). Scale bars represent 5 µm. Data were shown as mean ± SD of three independent experiments, * p≤0.05. (C) Mitochondria ultrastructure was analyzed by transmission electron microscopy. Scale bars represent 2 µm in the left panels and 100 nm in the right panels. The bracketed regions in the left panels are enlarged in the right panels.