Megakaryocytic Differentiation of K562 Cells Induced by PMA Reduced the Activity of Respiratory Chain Complex IV
Figure 2
Alterations in mitochondrial functions occured during PMA-induced K562 differentiation.
1×106 K562 cells were incubated with 5 nM PMA and harvested at 12 h, 24 h and 72 h respectively, stained with ROS fluorescence indicator MitoSOX (A), Fluo-3AM (B), and JC-1(C), followed by flow cytometry analysis. Data represented means ± SD obtained from three independent experiments, *p≤0.05. (D) O2 consumption by K562 cells during megakaryocytic differentiation were analyzed by ESR. (a): The K parameter curve. (b): Kinetic curve of O2 uptake by K562 cells. Graphics represented means ± SD obtained from three independent experiments. (E) Intracellular ATP level was measured using luminescence ATP detection assay system. Data represented means ± SD obtained from three independent experiments, *p≤0.05.