Fenofibrate Improves Renal Lipotoxicity through Activation of AMPK-PGC-1α in db/db Mice
Figure 8
PPARα, Phospho-AMPK Thr172, total AMPK, PGC-1α, and phospho-FoxO3a Ser253 and total FoxO3a levels in the renal medullar of the db/m and db/db mice with or without fenofibrate treatment.
Protein lysates (30 µg) from renal medullar were separated by SDS-PAGE and analyzed by Western blotting. A) Representative Western blotting is shown for PPARα, phospho-Thr172 AMPK, total AMPK, PGC-1α, phospho-FoxO3a Ser253, total FoxO3a, and β-actin. B-E) Quantitative analyses are shown for PPARα/β-actin (B), phospho-Thr172 AMPK/total AMPK (C), PGC-1α/β-actin (D), and phospho-FoxO3a Ser253 and total FoxO3a (E). *p<0.05 and **p<0.01 vs. the db/m control, db/m Feno, and db/db Feno mice.