PTK6 Promotes Cancer Migration and Invasion in Pancreatic Cancer Cells Dependent on ERK Signaling
Figure 5
The effect of ERK1/2 inhibition on PTK6-induced cellular migration and invasion.
A, Cells were treated with the specific ERK1/2 inhibitor, U0126 at 10 µM for 24 hours. ERK1/2 phosphorylation was inhibited and PTK6-induced activation of ERK1/2 was no longer detected in Panc1 or MIAPaCa2 cells. B, The effect of ERK1/2 inhibition on cellular migration (left) and invasion (right) ERK1/2 inhibition not only reduced basal migration and invasion in Panc1 and MIAPaCa2 cells, but abolished the effect of PTK6-overexpression on migration and invasion. *p<0.05 vs corresponding control with DMSO vehicle (by t-test). **p<0.05 vs PTK6-overexpressed cells with DMSO vehicle (by t-test). Each assay was performed in triplicate.