Identification of Restriction-Modification Systems of Bifidobacterium animalis subsp. lactis CNCM I-2494 by SMRT Sequencing and Associated Methylome Analysis
Figure 5
Restriction analysis of plasmid DNA.
Restriction analysis of total plasmid DNA isolated from three representative E. coli Xl1Blue-pWSK29-M.BanLI-M.BanLII transformants harbouring pDM1. Lane 1, molecular weight marker X (Roche). Total plasmid DNA from E. coli Xl1Blue -pWSK29-M.BanLI-M.BanLII + pDM1 restricted with Sal1, lanes 2–4 or AvaII, lanes 5–7. The sizes of the expected and obtained SalI restriction fragments for pWSK29-M.BanLI-M.BanLII component of the total plasmid preparation are 7019bp and 1353bp (and indicated in red) while for pDM1 the expected restriction fragment sizes are 3801bp and 2565bp (and indicated in blue). The expected SalI digest restriction fragment sizes for the total plasmid complement of E. coli Xl1Blue -pWSK29-M.BanLI-M.BanLII + pDM1 are indicated to the left of the figure.