Identification of Restriction-Modification Systems of Bifidobacterium animalis subsp. lactis CNCM I-2494 by SMRT Sequencing and Associated Methylome Analysis
Figure 4
(A). Restriction analysis of total DNA from B. animalis subsp. lactis CNCM I-2494. Lane1, molecular weight marker X (Roche). Lane 2: Unrestricted total DNA from B. animalis subsp. lactis CNCM I-2494, Lane 3–5 total B. animalis subsp. lactis CNCM I-2494 DNA restricted with lane 3, HindIII; lane 4, AvaII and lane 5, EcoRI. (B) Restriction analysis of plasmid DNA isolated from three representative E. coli Xl1Blue -pWSK29-M.BanLII. Plasmid DNA from E. coli Xl1Blue -pWSK29-M.BanLII restricted with PstI and XbaI, lanes 2–4 (expected and obtained, product sizes of PstI +XbaI digests are 5420 bp and 1605 bp), or AvaII, lanes 5–7.