Identification of Restriction-Modification Systems of Bifidobacterium animalis subsp. lactis CNCM I-2494 by SMRT Sequencing and Associated Methylome Analysis
Figure 2
Schematic representation of the E. coli-bifidobacterial shuttle vectors pDM1 and pDM2.
pDM1 is a derivative of pAM5 where the tetracycline resistance gene has been replaced with the spectinomycin resistance cassette. pDM2 is a derivative of the plasmid pDG7 where the spectinomycin resistance cassette was cloned in the unique EcoR1 and HindIII sites of pDG7.