Calorie Restriction Decreases Murine and Human Pancreatic Tumor Cell Growth, Nuclear Factor-κB Activation, and Inflammation-Related Gene Expression in an Insulin-like Growth Factor-1−Dependent Manner
Figure 4
Effect of silenced p65 on IGF-1-induced expression of downstream NF-κB targets in Panc02 cells.
A, Expression of p65 mRNA in Panc02 cells after exposure to either scrambled or p65-specific small interfering RNA. B, Ccdn1; Vegfa; Birc5; and COX-2 mRNA expression after 18-hour IGF-1 treatment (400 ng/mL). Bar graphs represent mean ± SEM (n = 3 separate experiments performed in triplicate for each gene). Different letters or asterisk denotes significance between groups. IGF-1, Insulin-like growth factor-1.