Calorie Restriction Decreases Murine and Human Pancreatic Tumor Cell Growth, Nuclear Factor-κB Activation, and Inflammation-Related Gene Expression in an Insulin-like Growth Factor-1−Dependent Manner
Figure 2
CR reduced Panc02 tumor volume and inflammatory profile.
CR decreased A, tumor growth and B, median tumor weight compared to CON mice (n = 15/group). Data shown are mean ± SD with the exception of the line in scatter plot representing the median tumor weight. C, CR reduced mRNA expression within the tumor microenvironment compared to CON mice (CON, n = 6; CR, n = 5). Data represent CR gene expression relative to CON. D, Mean fasting serum levels of MCP-1 (n = 8/group, randomly selected). E, Representative micrographs of p-p65-staining tumor sections (40X). F, Quantification of staining. Bar graph represents total percentage of p-p65-positive cells, with further illustration of intracellular localization (nuclear, black; cytoplasmic, white) (CON, n = 10 and CR, n = 7). Data shown represent mean ± SE. * denotes significant differences between CR and CON. CR, calorie restriction; CON, control diet; MCP-1, monocyte chemoattractant factor-1; p-p65, phosphorylated p65.