Interaction of 6 Mercaptopurine with Calf Thymus DNA – Deciphering the Binding Mode and Photoinduced DNA Damage
Figure 4
Competitive displacement assays.
(A) Fluorescence titration of CT-DNA and EB (intercalator) complex with 6MP. EB-DNA complex was excited at 471 nm and emission spectra were recorded from 500–700 nm. No change in fluorescence intensity was recorded with addition of increasing concentration of 6MP (B) Fluorescence titration of CT-DNA and AO (intercalator) complex with 6MP. CT-DNA-AO complex was excited at 471 nm and emission spectra were recorded from 500–600 nm. No change in fluorescence intensity was recorded with addition of increasing concentration of 6MP. (C) Fluorescence titration of CT- DNA and Hoechst (groove binder) complex with 6MP. Fluorescence intensity decreases with subsequent addition of 6MP. CT-DNA-Hoechst complex was excited at 343 nm and emission spectra were recorded from 360–600 nm.