The Hypothetical Protein ‘All4779’, and Not the Annotated ‘Alr0088’ and ‘Alr7579’ Proteins, Is the Major Typical Single-Stranded DNA Binding Protein of the Cyanobacterium, Anabaena sp. PCC7120
Figure 5
Quenching of fluorescence of native purified Anabaena SSB-like proteins compared with that of EcoSSB.
The quenching of fluorescence of (A) Alr0088, (B) Alr7579, (C) All4779 and (D) EcoSSB in the presence of 20 mM or 100 mM NaCl was expressed as a ratio of change in fluorescence (ΔF) and initial fluorescence (Fi). The (ΔF/Fi) was expressed as a function of ratio of concentrations of poly(dT) and protein. The horizontal lines indicate the point of saturation and the vertical lines drawn from the point of saturation indicate the probable length of ssDNA bound by one molecular unit of the protein.