Role of Corynebacterium glutamicum sprA Encoding a Serine Protease in glxR-Mediated Global Gene Regulation
Figure 5
In vitro digestion of MBP-GlxR by His6-SprA.
Protein purification and proteolytic assays were performed as described in the Materials and Methods. Proteins were incubated at 4°C or 30°C for 24 h, analyzed on a 15% SDS-PAGE gel, and then visualized by staining with Coomassie brilliant blue G250. Molecular weights are shown in kDa. MBP, maltose-binding protein; M, molecular weight markers; and OD, optical density.