Echicetin Coated Polystyrene Beads: A Novel Tool to Investigate GPIb-Specific Platelet Activation and Aggregation
Figure 5
Src-kinases and SYK are downstream effectors of echicetin beads induced platelet activation.
Washed human platelets were preincubated with Src-kinases inhibitor (PP2, 10 μM), an inactive analog (PP3, 10 μM) or Syk inhibitor (piceatannol, 10 μM) before stimulating with EP0.1 or vWF/R under stirring conditions. Representative aggregation curves of platelets stimulated with EP0.1 (A) or vWF/R (B) are shown. C, D. The graphs shows corresponding average values of aggregation from A and B. Platelets stimulated with EP0.1 (E) or vWF/R (F) were lysed at the indicated time and the phosphorylation of PKB, ERK and p38 was analyzed by Western blot. Total p38 served as loading control. Shown are representative blots of three independent experiments.+p<0.05 versus EP0.1.