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Pancreatic Alpha-Cell Dysfunction Contributes to the Disruption of Glucose Homeostasis and Compensatory Insulin Hypersecretion in Glucocorticoid-Treated Rats

Figure 2

The inhibition of glucagon secretion by pancreatic α-cells in response to glucose is impaired in DEX rats.

A: Glucagon secretion in response to 0.5 mmol/l or 11.1 mmol/l glucose in isolated islets from DEX and CTL rats (n = 10 wells). B: Total islet glucagon content (n = 10). C: In vitro glucagon secretion in response to 0.5 mmol/l or 11.1 mmol/l glucose in isolated islets that were pre-incubated for 3 h with 8.3 mmol glucose with or without 1 µmol/l dexamethasone (n = 10 wells). D: Total islet glucagon content (n = 10). Data are the mean ± SEM. * p<0.05 vs. CTL. p<0.05 vs. 0.5 mmol/l glucose. G, glucose.

Figure 2

doi: https://doi.org/10.1371/journal.pone.0093531.g002