Development of a T Cell Receptor Targeting an HLA-A*0201 Restricted Epitope from the Cancer-Testis Antigen SSX2 for Adoptive Immunotherapy of Cancer
Figure 2
Expression and biological function of SSX2 TCR-5, -8, -9 and -11.
A) Analysis of surface expression of SSX241-49-specific TCRs in CD8 and CD4 T cell populations by flow cytometry. Human peripheral blood mononuclear cells were stimulated with OKT3 and transduced with the indicated retroviral expression vectors. One week later, cells were stained with anti-human CD3, CD8 antibodies and with a fluorescently labeled tetramer containing the SSX241-49 peptide. Results from one representative donor of at least four independent experiments. Events gated on lymphoid, single, viable, CD3+ cells. B) – D) Concentration of IFNg in supernatants of TCR-transduced T cells cultured overnight with the indicated targets (1×105 effectors vs 1×105 targets). Results shown as average of duplicates for two representative donors out of four. UT: untranduced.