L-Arginine Stimulates Fibroblast Proliferation through the GPRC6A-ERK1/2 and PI3K/Akt Pathway
Figure 4
Effects of knockdown of GPRC6A on the activities of ERK, Akt, PKA and CREB in l-arginine-treated fibroblasts.
(A, B, C, D) Fibroblasts were treated with si RNA GPRC6A and then cells were stimulated with 6 mM l-arginine. The activities of ERK, Akt, PKA and CREB were analyzed by immunoblotting. Densitometry measurements for p-ERK, p-Akt, p-PKA and p-CREB were normalized to the amount of total ERK, Akt, PKA, and CREB, respectively. Results are presented as the fold change compared with the control group. Data are expressed as the mean ± SEM of 3 independent experiments (**p<0.01, Tukey’s post hoc test).