The Small Subunit 1 of the Arabidopsis Isopropylmalate Isomerase Is Required for Normal Growth and Development and the Early Stages of Glucosinolate Formation
Figure 7
Complementation analysis of Leu-auxotrophic E. coli strains.
Complementation capabilities of the different IPMI subunits were tested with the Leu-auxotrophic E. coli strains CV524 (ΔleuD, A, D) and CV522 (ΔleuC, B, C, E, F) on minimal medium with 1 mM IPTG and 2 mM Leu (D, E, F) at 37°C. The auxotrophic strains were transformed either with empty pUC19, leuC-leuD, or the different combinations of IPMI subunits leuC-SSU1/2/3, leuC*/LSU/LSU*-leuD or LSU*-SSU1/2/3. Untransformed bacteria were used as controls CV524 (ΔleuD) and CV522 (ΔleuC).