The Small Subunit 1 of the Arabidopsis Isopropylmalate Isomerase Is Required for Normal Growth and Development and the Early Stages of Glucosinolate Formation
Figure 4
Supplementation experiments with branched-chain amino acids.
(A to C) Col-0 wild-type (left parts of the images) and amiR-SSU1-B seedlings (line #9, right hand parts) were germinated and grown for 10 days on MS medium without exogenous amino acids (A, mock), in the presence of 0.2 mM Leu (B) and in the presence of 0.2 mM BCAA (C). (D to F) amiR-SSU1-B plants (line #9) were grown for 35 days on soil. Plants were watered without amino acids (D), with 2 mM Leu (E) or 2 mM BCAA (F).