Resveratrol Differentially Regulates NAMPT and SIRT1 in Hepatocarcinoma Cells and Primary Human Hepatocytes
Figure 4
Effects of FK866 and EX527 on p53 acetylation and cell viability in HepG2 cells.
Cells were stimulated with FK866 [10 nM] or EX527+TSA [20 µM EX527+1 µM TSA] in serum-free medium (con). Cells treated with A) FK866 and expression of acetylated p53 (K382) after 24 h. B) Cell viability of HepG2 cells after stimulation with FK866 for 48 h measured by WST-1 assay (n = 4). Data were normalised to serum-free medium (con) which was set 1 (**p<0.01; ***p<0.001 compared to serum free medium). C) Expression of acetylated p53 (K382), p21 protein and cleavage of caspase-3 were analysed in HepG2 cells treated with EX527+TSA for 24 h. GAPDH was used as loading control. One representative blot out of 3 independent experiments is shown.