Pharmacokinetics of Natural and Engineered Secreted Factors Delivered by Mesenchymal Stromal Cells
Figure 4
The immune system limits the bioavailability of MSC-derived IL-6.
Pharmacokinetic profile of sera IL-6 after IV cell administration. Approximately 1×106 MSCs were injected into C57Bl/6, Foxn1−/−, or NSG mice by IV injection. At different time points after cell injection, mice were sampled for blood plasma and serum human IL-6 levels were measured by ELISA. (B) AUC analysis of IL-6 exposure as a function of mouse strain. IV administration was significantly affected by mouse strain, particularly in severely immunodeficient mice, which had the highest exposure of IL-6 for a given cell mass. Data represent mean ± standard derivation of duplicate or triplicate experiments. Time points for serum analyses were 0.5, 8, and 24 hours after cell injection. Mice were serially analyzed as batches of N = 5 per study. * denotes P>0.01.