Cucurbitacin IIb Exhibits Anti-Inflammatory Activity through Modulating Multiple Cellular Behaviors of Mouse Lymphocytes
Figure 5
Effect of CuIIb on the activation of MAPKs in Con A-activated lymphocytes.
Cells were pretreated with CuIIb (10 µM) for 1 h, and then exposed to Con A (5 µg/mL) for 0, 15, 30, and 60 min, respectively. The phosphorylation of Erk1/2, JNK and p38 MAPKs at various time points was determined by Western blotting. β-Tubulin was used as a loading control. Representative western blots are shown in (A) and the relative densitometric ratios of each protein to β-tubulin are shown in (B). Arrow indicates a nonspecific band. Values are shown as mean ± SD of three experiments. *P<0.05; **P<0.01.