Molecular Cloning of a Novel Glucuronokinase/Putative Pyrophosphorylase from Zebrafish Acting in an UDP-Glucuronic Acid Salvage Pathway
Figure 5
Coupled HPLC Enzyme Assay of DrGK.
(A) Enzyme activity of DrGK was tested. Peak (3) indicates produced UDP-GlcA during coupled enzyme reaction. Peak (1) UMP, (2) AMP, (4) UDP, (5) ADP, (6) UTP and (7) ATP. (B) Control without DrGKUP. (C) 50 µM UDP-GlcA as reference compound.