Magnesium Inhibits Wnt/β-Catenin Activity and Reverses the Osteogenic Transformation of Vascular Smooth Muscle Cells
Figure 4
Magnesium inactivates Wnt/β-catenin signaling pathway in VSMC.
Relative mRNA expression on VSMC cultured with 3.3+1.4 mM Mg, and 3.3 mM P+1.4 mM Mg+10 µM 2-APB. Control group with 0.9 mM P+0.8 mM Mg was also included. (A) Frizzled 3, (B) DKK-1, (C) VCAN/versican, (D) cyclin D1, and (E) c-Myc. a, P<0.05 vs controls; b, P<0.05 vs VSMC with 3.3 mM P+0.8 mM Mg; and c, P<0.05 vs VSMC with 3.3 mM P+1.4 mM Mg. Bars are the mean±SE of three experiments in triplicate.